Journal: bioRxiv

Article Title: Oseltamivir (Tamiflu), a Commonly Prescribed Antiviral Drug, Mitigates Hearing Loss in Mice

doi: 10.1101/2024.05.06.592815

Figure Lengend Snippet: (A) Noise exposure and treatment schedule. Mice were exposed to 100 dB noise for 2 hours (8-16 kHz) and treated with oseltamivir for 3 days starting 24 hours following noise exposure. Cochleae were collected 4 days post noise insult. (B) Representative confocal images of cochlear cryosections stained with CD45 (red) and DAPI (blue). (C) Quantification of CD45 positive cells per cochlear section. Four treatment groups are carrier alone (black), oseltamivir alone (yellow), noise + carrier (red), and oseltamivir + noise (blue). Data shown as means ± SEM, **P<0.01 compared to noise alone by one-way ANOVA with Bonferroni post hoc test. n=3-6 mice.

Article Snippet: Tissues were stained overnight at 4°C with mouse CD45 antibody (1:50; Af114, R&D Systems).

Techniques: Staining

Journal: Frontiers in Pharmacology

Article Title: Jianpi Yangzheng Xiaozheng decoction alleviates gastric cancer progression via suppressing exosomal PD-L1

doi: 10.3389/fphar.2023.1159829

Figure Lengend Snippet: JPYZXZ reduced the number of MDSCs in tumor bearing mice. (A,B) Percentage of MDSCs (CD45 + CD11b+Gr1+) in blood determined using flow cytometry. (C,D) Percentage of MDSCs (CD45 + CD11b+Gr1+) in tumors determined using flow cytometry. (E) Immunofluorescence staining for DAPI (nucleus), CD11b, Gr-1, and PD-L1 in tumor tissue sections from the xenograft tumor model. Data are represented as the mean ± SEM, n = 5 mice or n = 3 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The single-cell suspensions (1 × 10 7 cells/mL) were stained with fluorochrome-coupled antibodies AF488-CD45 (Elabscience, E-AB-F1136L), PE/Dazzle™594-CD11b (BioLegend, 101256), and APC-Gr-1 (BioLegend, 108412) for 30 min at 4°C.

Techniques: Flow Cytometry, Immunofluorescence, Staining

Journal: Frontiers in immunology

Article Title: NLRP3 promotes allergic responses to birch pollen extract in a model of intranasal sensitization.

doi: 10.3389/fimmu.2024.1393819

Figure Lengend Snippet: FIGURE 4 Allergic sensitization to BPE is independent of inflammasome-induced caspase 1 activation. (A) Immunofluorescence staining of lung sections from C57BL/6 and Nlrp3-/- mice (Figure 3) against ASC (red) and CD45 (white). Dapi (blue) was used to stain cell nuclei. Scale bar is 20 µm. The dashed line marks the epithelial cell barrier. b= bronchiole. (B) Quantification of ASC+/CD45+ cells of 3 mice/group, 1 image/mouse as depicted in (A). (C) BPE-specific serum IgE was measured via antigen-induced cross-linking of Fcϵ-receptors on RBL-2H3 cells and subsequent degranulation and release of b-hexosaminidase (b-hex release). (D, E) Cytokines secreted into BALF (D) or by restimulated splenocytes (E) were measured by Multiplex technology. The data are presented as box plots of at least 5 mice per treatment group of 3 individual experiments. Each mouse is depicted as one data point. One-way ANOVA with Tukey’s post hoc test was performed to determine statistical significance. *p<0.05, **p<0.01.

Article Snippet: The following primary antibodies were used: ASC/TMS1 (D2W8U) Rabbit mAb (1:200, RRID: AB_2799736, Cell Signaling Technology), Goat Anti-Mouse CD45 Polyclonal antibody (1:100, RRID: AB_442146, R&D Systems).

Techniques: Activation Assay, Staining, Multiplex Assay