Journal: bioRxiv

Article Title: Oseltamivir (Tamiflu), a Commonly Prescribed Antiviral Drug, Mitigates Hearing Loss in Mice

doi: 10.1101/2024.05.06.592815

Figure Lengend Snippet: (A) Noise exposure and treatment schedule. Mice were exposed to 100 dB noise for 2 hours (8-16 kHz) and treated with oseltamivir for 3 days starting 24 hours following noise exposure. Cochleae were collected 4 days post noise insult. (B) Representative confocal images of cochlear cryosections stained with CD45 (red) and DAPI (blue). (C) Quantification of CD45 positive cells per cochlear section. Four treatment groups are carrier alone (black), oseltamivir alone (yellow), noise + carrier (red), and oseltamivir + noise (blue). Data shown as means ± SEM, **P<0.01 compared to noise alone by one-way ANOVA with Bonferroni post hoc test. n=3-6 mice.

Article Snippet: Tissues were stained overnight at 4°C with mouse CD45 antibody (1:50; Af114, R&D Systems).

Techniques: Staining

Journal: PLoS ONE

Article Title: Fibrogenic Potential of Human Multipotent Mesenchymal Stromal Cells in Injured Liver

doi: 10.1371/journal.pone.0006657

Figure Lengend Snippet: A) Fibroblast-like morphology of adult (a) and pediatric (b) MSC. Both cell types showed similar morphology. B) Flow cytometry of cells stained for CD34, CD36, CD44, CD45, CD54, CD90, CD105, CD106, HLA ABC and isotype controls showing similar expression of surface antigens in adult (a) and pediatric (b) MSC. A representative example of adult and pediatric MSC is shown. Red histograms: specific marker; unfilled black histograms: isotype controls. C) Adipogenic differentiation was induced with adipocyte differentiation medium. After 3 weeks aMSC (a) and pMSC (b) were fixed and stained with Oil-red-O. In control medium (Insets), very few cells contained lipid droplets. In adipogenic differentiation medium a majority of cells contained lipid droplets stained in red. D) Chondrogenic differentiation was induced in MSC pellet culture in chondrogenic differentiation medium. After 4 weeks, pellets were fixed and sections were analyzed using Masson trichrome staining (a,b blue) and anti-collagen type II immunohistochemistry (c,d red). The images represent zoomed portion (delimitated by white frames) of the picture shown in the insets. The pellet structure appeared compact and contained abundant collagen fibrils. E) Osteogenic differentiation was induced by culturing aMSC (a) and pMSC (b) in osteogenic differentiation medium. After 4 weeks, cells were washed and incubated with Fast red and Sodium alpha-naphtyl phosphate solutions for 30 min at 4°C. Nuclei were stained with hemalun. MSC expressing alkaline phosphatase showed brown colored cytoplasm and displayed typical star-shaped cell morphology. In control medium (Insets), only few cells showed alkaline phosphatase expression.

Article Snippet: Following antibodies (Ab) were used for fluorescent activated cell sorting (FACS) analysis and immunohistochemistry: mouse anti-CD11b (Hycult biotechnology, Uden, the Netherlands), mouse anti-CD31 (Dako, Baar, Switzerland), phycoerythrin (PE)-conjugated mouse anti-CD34, Fluorescein isothiocyanate (FITC)-conjugated mouse anti-CD36, PE-mouse anti-CD44, PE-mouse anti-CD54, mouse anti-CD90, mouse anti-CD106, mouse isotype control (all from Becton Dickinson, Basel, Switzerland), PE-mouse anti-CD45 (R&D systems, Abingdon, UK), FITC-mouse anti-CD105 (Serotec, Oxford, UK), mouse anti-HLA-ABC (Chemicon Australia, Victoria, Australia), mouse anti-vimentin (Dako, Baar, Switzerland), mouse anti-human serum albumin, mouse anti-beta cytoplasmic actin (both from Sigma, Buchs, Switzerland), rabbit anti-human collagen type II (Mono-San, Uden, The Netherlands) and mouse anti-human alpha smooth muscle actin Ab .

Techniques: Flow Cytometry, Staining, Expressing, Marker, Immunohistochemistry, Incubation